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Fig. 3 | Breast Cancer Research

Fig. 3

From: Consistent and reproducible cultures of large-scale 3D mammary epithelial structures using an accessible bioprinting platform

Fig. 3

Organoid fusion occurs between organoids printed up to 500 μM apart. (a) MCF12A cells were printed (40 cells per print location) at distances of 500 μm, 400 μm, 300 μm, and 200 μm (columns left to right, respectively). Consistent fusion was seen by day 11 in all cases, and contiguous organoids formed between prints spaced not more than 400 μm apart. Scale bar: 500 μm. (b) By day 14, organoids fused into contiguous luminal structures that maintained the linear array shape. Scale bar: 500 μm. (c) 4′,6-diamidino-2-phenylindole (DAPI) stained 5 μM section of a large contiguous, duct-like structure in 21-day-old MCF12A culture. Scale bar: 200 μm

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Breast Cancer Research

ISSN: 1465-542X

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