Fig. 5

Collagen XIII enhances cancer cell invasion and stemness through β1 integrin. a Immune fluorescence imaging analysis of β1 integrin activation in control and Col13^−/− MDA-MB-231 cells (left). Bar graph quantifying the foci number of activated β1 integrin staining on cell membrane in control and Col13^−/− MDA-MB-231 cells (right). Data are represented as the mean ± s.e.m.; n = 10 (fields); ** p < 0.01, *** p < 0.001. Scale bar: 5 μm. b Immune fluorescence imaging analyzed β1 integrin activation in control and Col13-expressing MCF-10A cells (left). Bar graph quantifying the foci number of activated β1 integrin staining on cell membrane in control and Col13-expressing MCF-10A cells (right); n = 10 (fields); ** p < 0.01. Scale bar: 5 μm. c The velocity (left) and path length (right) of single cell migration were quantified in Col13-expressing MCF-10A cells in the presence of β1 integrin functional blocking antibody AIIB2 or control IgG; n = 27; *** p < 0.001; n.s., no significance. d Bar graph quantifying invasion of Col13-expressing MCF-10A cells in the presence of AIIB2 or IgG; n = 3; ** p < 0.01. e Bar graph quantifying the mammosphere forming efficiency of Col13-expressing MCF-10A cells in the presence of AIIB2 or IgG; n = 3; * p < 0.05. f Western blot data showing that AIIB2 treatment enhanced PARP-cleavage in Col13-expressing MCF-10A cells