Platelet basal activity and ADP-induced reactivity in orthotopic murine 4T1 breast cancer model. Platelet basal activation and their ADP-induced reactivity assessed as percentage of platelet surface expression of P-selectin (a), active form of receptor GPIIb/IIIa (b), von Willebrand factor (vWF) binding (c), and fibrinogen binding (d) (see Methods), presented as median and IQR. Black circle indicates outlier. Data analyzed with Kruskal–Wallis test followed by Dunn’s multiple comparison test since they either did not display normal distribution and/or their variances were heterogeneous. a n = 10, n = 7, n = 9, n = 7, n = 8, and n = 12 for healthy control, 1st, 2nd, 3rd, 4th, and 5th week, respectively; and n = 9, n = 7, n = 8, n = 6, n = 6, and n = 12 for healthy control, 1st, 2nd, 3rd, 4th, and 5th week after ADP stimulation. b n = 10, n = 7, n = 9, n = 7, n = 8, and n = 13 for healthy control, 1st, 2nd, 3rd, 4th, and 5th week, respectively; and n = 10, n = 7, n = 9, n = 7, n = 7, and n = 13 for healthy control, 1st, 2nd, 3rd, 4th, and 5th week after ADP stimulation. c n = 10, n = 7, n = 8, n = 7, n = 8, and n = 18 for healthy control, 1st, 2nd, 3rd, 4th, and 5th week, respectively; and n = 10, n = 7, n = 9, n = 7, n = 7, and n = 19 for healthy control, 1st, 2nd, 3rd, 4th, and 5th week after ADP stimulation. d n = 10, n = 7, n = 8, n = 7, n = 8, and n = 20 for healthy control, 1st, 2nd, 3rd, 4th, and 5th week, respectively; and n = 9, n = 7, n = 9, n = 7, n = 8, and n = 20 for healthy control, 1st, 2nd, 3rd, 4th, and 5th week after ADP stimulation. Statistical significance at level of *P < 0.05, **P < 0.01, and ***P < 0.001