Fig. 3From: Pyruvate carboxylase supports the pulmonary tropism of metastatic breast cancerDepletion of the PC inhibits in vitro cell growth. Two different shRNA sequences targeting PC (shPC25 and shPC28) were stably expressed in the 4 T1 cells and cellular viability was assessed under two-dimensional (a and c) and three-dimensional (b and d) culture conditions containing high (4.5 mg/ml; a and b) or low (1 mg/ml; c and d) amounts of glucose. Data are the mean ± SE of the three separate experiments completed in triplicate resulting in the indicated P valuesBack to article page