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Fig. 5 | Breast Cancer Research

Fig. 5

From: Estrogen promotes estrogen receptor negative BRCA1-deficient tumor initiation and progression

Fig. 5

Estrogen activates the Akt pathway in Brca1-deficient mammary tumors. a, b Representative p18−/−;Brca1MGKO tumors treated with E2 or placebo were analyzed by immunohistochemical staining (IHC) (a) and western blot (b). H-scores for p-Akt and p-4E-bp1 in a were calculated in four randomly selected fields for each section of a tumor, and the results represent the mean ± SD of three individual tumors per group. c p18−/−;Brca1MGKO tumor cells were treated with DMSO or E2, and the expression of p-Akt, p-mTor, p-Gsk3β and p-4E-bp1 was analyzed by western blot. d BRCA1 mutant patient-derived xenograft (PDX) tumors treated with E2 or placebo were analyzed by IHC. H-scores for p-Akt and p-4E-bp1 were calculated in four randomly selected fields for each section of a tumor, and the results represent the mean ± SD of three individual tumors per group. e Representative BRCA1 mutant PDX tumors treated with E2 or placebo were analyzed by western blot

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