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Table 1 Antibodies used for immunolabeling

From: Syndecan-1 induction in lung microenvironment supports the establishment of breast tumor metastases

Antigen

Dilution

Specificity

Catalog number

Company/source

CD31

1:400

Goat anti-mouse

AF3628

R&D Systems, Minneapolis, MN, USA

Ki67

1:1000

Mouse anti-human, clone MIB-1

M7240

Dako, Santa Clara, CA, USA

Ki67

1:200

Rabbit anti-mouse, clone D3B5

12,202

Cell Signaling Technology, Danvers, MA, USA

αSMA

1:500

Rabbit anti-mouse

ab5694

Abcam, Cambridge, MA, USA

F4/80

1:400

Rabbit anti-mouse

ab100790

Abcam

ER-TR7

1:200

Rat anti-mouse

ab51824

Abcam

CD45

1:200

Rabbit anti-mouse

ab10558

Abcam

CD68

1:200

Rat anti-mouse

MCA1957T

BioRad

Cleaved Caspase-3

1:200

Rabbit anti-mouse, D175

9661

Cell Signaling Technology

Sdc-1

1:200

Rat anti-mouse

NA

gift from Dr. Rapraeger

Sdc-1

1:100

Mouse anti-human, clone B-B4

MCA681H

Serotec

Vimentin

predilute

Mouse anti-human

790–2917

Ventana Medical Systems, Tucson, AZ, USA

CD4

1:200

Rabbit anti-mouse

ab221775

Abcam

CD8

1:750

Rabbit anti-mouse

ab209775

Abcam