From: Syndecan-1 induction in lung microenvironment supports the establishment of breast tumor metastases
Antigen | Dilution | Specificity | Catalog number | Company/source |
---|---|---|---|---|
CD31 | 1:400 | Goat anti-mouse | AF3628 | R&D Systems, Minneapolis, MN, USA |
Ki67 | 1:1000 | Mouse anti-human, clone MIB-1 | M7240 | Dako, Santa Clara, CA, USA |
Ki67 | 1:200 | Rabbit anti-mouse, clone D3B5 | 12,202 | Cell Signaling Technology, Danvers, MA, USA |
αSMA | 1:500 | Rabbit anti-mouse | ab5694 | Abcam, Cambridge, MA, USA |
F4/80 | 1:400 | Rabbit anti-mouse | ab100790 | Abcam |
ER-TR7 | 1:200 | Rat anti-mouse | ab51824 | Abcam |
CD45 | 1:200 | Rabbit anti-mouse | ab10558 | Abcam |
CD68 | 1:200 | Rat anti-mouse | MCA1957T | BioRad |
Cleaved Caspase-3 | 1:200 | Rabbit anti-mouse, D175 | 9661 | Cell Signaling Technology |
Sdc-1 | 1:200 | Rat anti-mouse | NA | gift from Dr. Rapraeger |
Sdc-1 | 1:100 | Mouse anti-human, clone B-B4 | MCA681H | Serotec |
Vimentin | predilute | Mouse anti-human | 790–2917 | Ventana Medical Systems, Tucson, AZ, USA |
CD4 | 1:200 | Rabbit anti-mouse | ab221775 | Abcam |
CD8 | 1:750 | Rabbit anti-mouse | ab209775 | Abcam |