Fig. 5
From: Syndecan-1 induction in lung microenvironment supports the establishment of breast tumor metastases
Effect of ambient temperature on metastatic efficiency. A subset of animals was moved to a housing environment with a higher, thermo-neutral temperature of approximately 31 °C, 2 weeks prior to inoculation and maintained at that temperature throughout the duration of the experiment. The 4T1 tumor cells (1 × 107 tumor cells in 10 μL serum-free DMEM) were then injected into the fat pads of Sdc1+/+ and Sdc1−/− mice. The animals were sacrificed 30 days after tumor cell inoculation and lung sections were examined for metastases. Shown are numbers of metastases per mouse (one outlier data point of 18 metastases per mouse in Sdc1−/−; 21 °C group is off scale but is included in mean and SD calculation)