Fig. 1

Effect of host syndecan-1 (Sdc1) on metastatic efficiency of 4T1 mouse mammary carcinoma cells. The 4T1 tumor cells (1 × 10⁷ cells in 10 μL serum-free DMEM) were injected into the exposed 4th mammary gland as described in “Methods” and mice were sacrificed after 30 days. a Small, early metastasis, cuffing blood vessel in lung (M, metastasis; V, vessel; L, lung parenchyma; original magnification × 400; scale bar indicates 100 μm). b Number of metastatic lesions per mouse. Metastases were counted on single histologic sections of both lungs. Bars indicate mean +/− standard deviation. c Metastatic tumor burden expressed as percent lung tissue occupied by metastatic lesions (see “Methods” for details). d Average area of metastatic lesions expressed in pixels as measured on histologic sections