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Fig. 1 | Breast Cancer Research

Fig. 1

From: SNAI2 upregulation is associated with an aggressive phenotype in fulvestrant-resistant breast cancer cells and is an indicator of poor response to endocrine therapy in estrogen receptor-positive metastatic breast cancer

Fig. 1

Upregulation of SNAI2 in endocrine-resistant cells compared to parental endocrine-sensitive cell lines. Evaluation of the gene expression of epithelial-mesenchymal transition (EMT) transcription regulators in MCF-7-based fulvestrant-resistant (FulvR) vs. fulvestrant-sensitive (a) and tamoxifen-resistant (TamR) vs. tamoxifen-sensitive (b) cells by gene array. Verification of altered expression of SNAI2 in four fulvestrant-resistant (c) and four tamoxifen-resistant cell lines (d), by reverse transcription (RT)-quantitative (q)PCR (RT-qPCR). Gene expression was normalized using PUM1 and depicted as the relative expression in endocrine-resistant vs. endocrine-sensitive cells: *p < 0.05. Data are shown with error bars representing mean ± standard deviation. SNAI2 protein expression alteration in the same fulvestrant-resistant (e) and tamoxifen-resistant cell lines (f), as determined by western blotting. β-actin was used as loading control. A representative of three independent experiments is shown

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