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Fig. 3 | Breast Cancer Research

Fig. 3

From: Correction to: Buthionine sulfoximine sensitizes antihormone-resistant human breast cancer cells to estrogen-induced apoptosis

Fig. 3

Buthionine sulfoximine (BSO) plus estradiol induce apoptosis in MCF-7:2A cells. a Terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling (TUNEL) staining was used to detect apoptosis in MCF-7 (top panel) and MCF-7:2A cells (bottom panel) following BSO plus 17β-estradiol (E2) treatment for 96 h. The Click-iT™ Plus TUNEL kit (Thermo Fisher Scientific) was used instead of the TUNEL In Situ Cell Death kit conjugated with horse-radish peroxidase (POD) (Roche Applied Science, Indianapolis, IN, USA) as previously described in the original publication [1]. TUNEL-positive cells were stained red and the nuclei were counterstained with DAPI (blue). Merged images are shown at right with overlapping DAPI in blue and Alexa Fluor 594 in red. Slides were photographed through a fluorescence microscope under 20× magnification. Average intensity of TUNEL signal was taken from 3 images quantified using red color channel on Image J. Bar graphs (right) quantify apoptosis (TUNEL-positive cells), which is expressed as percentage of apoptotic cells for each condition. Data are presented as mean ± standard error (n = 3). Mean ± SEM); *, p < 0.001 significantly different from control cells; #, p < 0.01 significantly different from E2 or BSO alone

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