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Fig. 3 | Breast Cancer Research

Fig. 3

From: Targeting tumour re-wiring by triple blockade of mTORC1, epidermal growth factor, and oestrogen receptor signalling pathways in endocrine-resistant breast cancer

Fig. 3

Anti-proliferative effect of RAD001 (RAD), neratinib (Ner), or their combination in endocrine-resistant and -sensitive BC cell lines. Cell lines were treated with vehicle or sub-optimal concentrations for each drug alone or in combination, both in the absence and presence of 0.01 nM exogenous E2. After 6 days of treatment, cell viability was analysed using cell titer-glo and data expressed as fold-change relative to vehicle control. Error bars represent mean ± SEM. *p < 0.05; **p < 0.01; ***p < 0.001. Concentrations used in dextran charcoal (DCC): wt-MCF7 (0.75 nM RAD001, 2000 nM neratinib); MCF7-LTED (0.75 nM RAD001, 500 nM neratinib); wt-SUM44 (0.75 nM RAD001, 2000 nM neratinib); SUM44-LTED (0.4 nM RAD001, 500 nM neratinib); wt-HCC1428 (12.5 nM RAD001, 1200 nM neratinib); HCC1428-LTED (3 nM RAD001, 250 nM neratinib). Concentrations used in E2: wt-MCF7 (1.5 nM RAD001, 200 nM neratinib); MCF7-LTED (1.5 nM RAD001, 300 nM neratinib); wt-SUM44 (0.37 nM RAD001, 450 nM neratinib); SUM44-LTED (0.37 nM RAD001, 250 nM neratinib); wt-HCC1428 (1.5 nM RAD001, 500 nM neratinib); HCC1428-LTED (3 nM RAD001, 250 nM neratinib)

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