Fig. 4

Malignant breast cancer evolved from benign nodules in BCCIP knockdown mice. a Representative mammary gland tumor evolved from a benign nodule (after 11 months of the initial detection of nodule). b, c HE staining of representative tumor dissected from BCCIP-CON (b) and BCCIP-CKD (c) mice. d, e Representative images from immunohistochemical analysis of mBCCIP status on formalin-fixed paraffin-embedded sections of mammary tumors from BCCIP-CON (d) and BCCIP-CKD (e). f, g IHC showing p16 (CDKN2A) in a benign lesion (f) and a malignant tumor (g) formed in BCCIP knockdown mice. h, i 53BP1 expression in malignant tumor evolved from benign nodules of BCCIP knockdown mice (i) and a de-novo tumor of wild-type mice (h). BCCIP BRCA2 and CDKN1A interacting protein, HE hematoxylin and eosin, IHC immunohistochemistry, CKD FVB:LoxPshBCCIP ^+/− ;K14-Cre ^+/−, CON FVB:LoxPshBCCIP ^+/− ;K14-Cre ^−/−