Fig. 3From: Calmodulin-like protein 3 is an estrogen receptor alpha coregulator for gene expression and drug response in a SNP, estrogen, and SERM-dependent fashionEffect of SNP-dependent regulation of CALML3 and ERĪ± on the expression of ZNF423. a, b Expression of ZNF423 and BRCA1 determined by qRT-PCR after knocking down CALML3 in ERĪ±ā+āZR75-1 breast cancer cells with ZNF423 WT or variant genotypes. mRNA expression levels shown as the mean of three independent experiments (Ā± SEM), comparisons made by two-tailed Studentās t test. Protein levels were determined by western blot analysis. c Schematic representation shows the role of CALML3 in the SNP-dependent effect of the ER dimer. d, e ChIP-qPCR assays performed using CALML3 antibody after knocking down ERĪ± (d) or vice versa (e) in CRISPR-ZR75-1 cells (WT) and ZR75-1 cells (variant) treated with E2āĀ±ā4-OH-TAM/raloxifen. Primer locations indicated in (c). Enrichment of DNA fragments (three independent experiments, meanāĀ±āSEM) between different treatments was compared by two-tailed Studentās t test. *pā<ā0.05, **pā<ā0.01, ***pā<ā0.001, ****pā<ā0.0001. 4-OH-TAM 4-hydroxytamoxifene, E2 estradiol, ERĪ± estrogen receptor alpha, ERE estrogen response element, Ral raloxifene, V variant, WT wild typeBack to article page