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Fig. 3 | Breast Cancer Research

Fig. 3

From: Effect of neoadjuvant chemotherapy on tumor-infiltrating lymphocytes and PD-L1 expression in breast cancer and its clinical significance

Fig. 3

Comparison of PD-L1 expression prior to (pre-neoadjuvant) and following (post-neoadjuvant) neoadjuvant treatment. Changes in PD-L1 QIF scores in (a) the tumor compartment and (b) the stromal compartment from pre-chemotherapy biopsy to surgical specimen (Mann–Whitney test, mean and SEM). c Representative QIF images (400× magnification, insert 100×) of matched pre-neoadjuvant (left column) and post-neoadjuvant (right column). Upper row: case with high PD-L1 expression in chemo-naive biopsy that displays low PD-L1 following treatment. Middle row: case with low PD-L1 expression prior to and increased expression in the residual tumor. Lower row: case with low PD-L1 expression before and after treatment. DAPI counterstaining in blue, cytokeratin tumor mask in green, and PD-L1 in red. d Distribution of PD-L1 expression in the stroma and tumor in 41 matched cases with pre-neoadjuvant and post-neoadjuvant specimens. Colors correspond to compartment (red for tumor and stroma, green for tumor only, purple for stroma only, and blue for PD-L1 negative cases) and numbers in the column show the number of cases for each condition. PD-L1 programmed death ligand 1, DAPI 4,6-diamidino-2-phenylindole (Color figure online)

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