Fig. 1From: Interaction with adipocyte stromal cells induces breast cancer malignancy via S100A7 upregulation in breast cancer microenvironmentCarcinoma–adipose stromal cell (ASC) interaction alters characteristics of ASCs and promotes proliferation and migration rate of breast cancer cells. a Reduction of adipocyte transcription markers in human primary adipocyte stromal cells (hASCs) and 3T3-L1 adipocyte stromal cells (3T3a) by coculture with MCF7 cells, from those levels in preadipocytes. Pre-hASCs Primary human preadipocytes. * P < 0.0001 compared with basal preadipocytes; ** P < 0.05 compared with hASC + MCF7. b Cell proliferation rate by WST-8 assay in MCF7, T47D, SK-BR-3, and ZR-75-1 cells by treatment of conditioned medium (CM) derived from each cell line, pre-3T3-L1, and 3T3a for 4 days. CCM Breast cancer cell-derived conditioned medium, pre-CM Preadipocyte-derived conditioned medium, ACM 3T3a adipose stromal cell-derived conditioned medium. * P < 0.05; ** P < 0.0001 compared with control. c Coculture with ASCs (+3T3a) significantly induced migration rate of MCF7, SK-BR-3, T47D, and ZR-75-1 cells compared with monocultured cells. * P < 0.05 compared with monoculture. C/EBPα CCAAT/enhancer-binding protein α, FABP4 Fatty acid-binding protein 4, mRNA Messenger RNA, PPARγ Peroxisome proliferator-activated receptor-γBack to article page