Fig. 8

JMJD6 expression affects SMAD phosphorylation. Immunoblots show that the level of total SMAD2/3, phosphorylated SMAD2 and phosphorylated SMAD3 are decreased in MCF-7 J1-OE clones, and increased in JMJD6 siRNA-mediated knock-down in MCF-7 and MDA-MB231. The numbers next to the SMAD2P/3P denote the expected sites of phosphorylation detected by the antibodies. N.S. denotes non-specific bands from the blots which indicate equal loading of the protein lysates