Fig. 2
Electrochemical analysis of reactive oxygen species/reactive nitrogen species in response to stress hormones. MCF-7 (a) and MDA-MB-231 (b) cells were incubated with cortisol (Cort) (1 μM) and RU486 (1 μM). MCF-7 (c) and MDA-MB-231 (d) were also incubated with norepinephrine (NE) (1 μM), propranolol (1 μM) and non-specific and specific nitric oxide synthase blockers (NG-nitro-L-arginine methyl ester (L-NAME) (100 μM) and 1400 W(10 μM)) for 30 minutes prior to 30 minutes exposure to stress hormone. Levels of hydrogen peroxide (H 2 O 2 ) and nitrogen dioxide (NO 2 ) were measured using electrochemical sensors. Data are mean ± SEM and statistical significance was determined using one-way analysis of variance (post hoc Tukey multiple comparisons). *Significant increase compared to unstimulated cells: *p < 0.05, **p < 0.01, ***p < 0.001