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Fig. 4 | Breast Cancer Research

Fig. 4

From: Zoledronic acid alters hematopoiesis and generates breast tumor-suppressive bone marrow cells

Fig. 4

Zoledronic acid (ZOL) generates tumor-suppressive bone marrow cells. a Experimental design to test bone marrow tumor support function. Bone marrow cells (BMCs) were harvested from control (Ctl)-treated or ZOL-treated donor mice 3 and 10 days (nude) or 5 and 15 days (C57Bl/6) after treatment (n = 3 donor mice/cohort): 7.5 × 105 BMCs were directly admixed with 2.5 × 105 luciferase + MDA-MB-231 BO2F11 bone tropic human breast cancer cells and admixtures immediately injected subcutaneously into cohorts of recipient nude mice (n = 3–6 recipient mice per cohort with bilateral subcutaneous injections; b and c are representative images of one of three biological replications). Tumor growth was monitored over a 14-day experimental time course. b Average radiance signal at each injection site per cohort at experimental endpoint (day (d)14) resulting from admixtures of MDA-MB-231 BO2F11 tumor cells with BMCs from indicated Ctl-treated or ZOL-treated donor nude mice; *p < 0.05. Table indicates incidence of tumor formation resulting from admixtures of breast tumor cells with BMCs from indicated donor mice. c Representative In vivo imaging system images of luciferase + MDA-MB-231 BO2F11 tumors resulting from admixtures with BMCs from Ctl-treated or ZOL-treated donor nude mice (10 days after treatment of donor mice). Imaging was acquired at the 14-day experimental end point. d Nude mice were treated with 100 μL of vehicle or 100 μg/kg ZOL; 3 days later, mice were injected subcutaneously with 2.5 × 105 MDA-MB-231 BO2F11 and tumor growth was monitored over a 14-day time period. Average tumor volume (mm3) for indicated cohorts is shown; n.s. not statistically significant. e Nude mice were injected orthotopically with MDA-MB-231 bone-tropic cells and tumors grew for 30 days before mice were given one dose of Ctl treatment or ZOL (100 μg/kg). BMCs were harvested 3 days after Ctl or ZOL administration and Lin-Sca1+cKit+(LSKs) were analyzed by flow cytometry, *p < 0.05

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