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Fig. 5 | Breast Cancer Research

Fig. 5

From: CCL2-driven inflammation increases mammary gland stromal density and cancer susceptibility in a transgenic mouse model

Fig. 5

The effect of CCL2 overexpression on abundance of stroma and collagen. Sections of mammary gland tissue from control (a, d, f) and Mmtv-Ccl2 (b, e, h) mice at proestrus were stained with H&E (a, b), Masson’s trichrome (c, d) and collagen I antibody (g, h) and quantified (c, f, i); n = 6 per group. Data are presented as mean + SEM with statistical analysis conducted using the unpaired t test, *p < 0.05 compared to control. Mammary glands from both groups of mice (n = 8) were dissected and frozen in liquid nitrogen. Messenger RNAs of collagen remodelling enzymes including Lox, Mmp2, Mmp9, Timp1, Timp2 and Timp3 were extracted and measured by RT-PCR. The amount of mRNA was normalised to Actb expression, and is given in arbitrary units, where the average of the control is 1 (j). Data are presented as mean + SEM with statistical analysis conducted using the unpaired t test; *p < 0.05 compared to control. LOX lysyl oxidase, MMP matrix metalloproteinase, TIMP tissue inhibitor of matrix metalloproteinases

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