Fig. 3From: CCL2-driven inflammation increases mammary gland stromal density and cancer susceptibility in a transgenic mouse modelThe effect of CCL2 overexpression on mammary gland morphogenesis during ovarian cycle. Mammary glands from control and Mmtv-Ccl2 mice were whole-mounted and stained with carmine alum at estrus (a, e), metestrus (b, f), diestrus (c, g) and proestrus (d, h), respectively. The number of branch points per millimetre was calculated (k). Sections of paraffin-embedded mammary glands from control and Mmtv-Ccl2 mice at the four stages of the cycle were H&E-stained (i, j, respectively, only proestrus stage shown) and alveolar epithelium quantified (l); n = 6–9 per group. Data are presented as mean + SEM with statistical analysis conducted using the unpaired t test, *p < 0.05Back to article page