Fig. 1

Generation of mouse mammary tumour virus 206 transgenic mice (Mmtv-Ccl2). The Mmtv-Ccl2 expression cassette was detected in three of twenty-nine mice by PCR screening and the expression of both endogenous genomic and cloned Ccl2 mRNA in different tissues was measured by RT-PCR with product sizes of 451 bp and 125 bp, respectively (a). CCL2 protein detected by immunohistochemistry in the mammary gland from female offspring from founder mouse #29 (b) compared to non-transgenic control (c). RNA encoding MMTV (d) and CCL2 (e), and CCL2 protein (f) were quantified in spleen (Sp), kidney (Kid), ovary (Ov), liver (Liv), salivary gland (SG) and mammary gland (MG) from Mmtv-Ccl2 and non-transgenic control mice. Abundance of mRNA was normalised to Actb expression, and is given in arbitrary units where the average of the non-transgenic mammary gland control is 1; n = 5 per group. Data are presented as mean + SEM with statistical analysis conducted using the unpaired t test, *p < 0.05