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Fig. 4 | Breast Cancer Research

Fig. 4

From: Thymosin beta 10 is a key regulator of tumorigenesis and metastasis and a novel serum marker in breast cancer

Fig. 4

Thymosin beta 10 (TMSB10) promotes lung metastasis in vitro and in vivo. a In wound healing assays, overexpression of TMSB10 significantly increased the migration ability of the indicated cells, while downregulation of endogenous TMSB10 reduced the migration ability of the indicated cells. b Overexpression of TMSB10 increased, while downregulation of TMSB10 reduced, the migration ability of the indicated cells in the migration assay. Each bar represents the mean values ± SD of three independent experiments. *P < 0.05. c Overexpression of TMSB10 increased, while downregulation of TMSB10 reduced, the invasive ability of the indicated cells in the invasion assay. Each bar represents the mean values ± SD of three independent experiments. *P < 0.05. d In vivo metastasis assays of BT-549 cells with TMSB10 overexpression or knockdown. Bioluminescent imaging (BLI) was used to weekly monitor the lung metastasis burden of xenografted animals. Shown are BLI images of representative mice on days 0 and 35 after injection, respectively. The color scale depicts the photon flux (photons per second) emitted from the metastatic cells. e Kaplan-Meier survival curves for mice. f and g Lung metastases in mice were confirmed by H&E staining. Arrows indicate the metastatic colonization of tumor cells (left panel). The numbers of lung tumor nests in each group was counted under a low power field and are presented as the median values ± quartile values (right panel). *P < 0.05

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