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Fig. 4 | Breast Cancer Research

Fig. 4

From: Naringenin prevents TGF-β1 secretion from breast cancer and suppresses pulmonary metastasis by inhibiting PKC activation

Fig. 4

Naringenin (Nar) improves the immune activities of T cells. a Flow cytometry analysis of T-cell activation marker expressed CD4+CD44+CD62L cells in lymphocytes purified from spleen and lung tissues. b Flow cytometry analysis of the percentage of Gr-1+CD11b+ cells (MDSC) in leukocytes purified from spleen and lung tissues. Data are from three mice per group. c Analysis of IFNγ+ specific CD8+ cell percentage in lymphocytes purified from the spleen of mice bearing 4T1/RFP tumors or 4T1/TGF-β1 tumors. d Analysis of the mRNA level of IFNγ and granzyme-B (Gran B) in lung samples of mice bearing 4T1/RFP tumors or 4T1/TGF-β1 tumors. Data are from three experiments. *P <0.05, **P <0.01, ***P <0.001. Error bars indicate SE. e, f Bioluminescence imaging of the dissected lungs e and whole bodies f of nude mice after transferring T cells from Balb/c mice treated with or without naringenin for 28 days using the IVIS system (n = 6). g Incidence of pulmonary metastasis of the dissected lungs and whole bodies (n = 6). CMCNa sodium carboxyl methyl cellulose, IFN interferon, TGF transforming growth factor

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