Fig. 7

Celecoxib diminishes collagen deposition in PyMT mammary tumors, but does not affect tumor-free mammary glands. a, b Masson’s trichrome stain of mammary tumors or mammary glands of nulliparous mice treated with vehicle or celecoxib. Collagen fibers are in blue, cell nuclei are black and the cell cytoplasm, muscle tissue, and erythrocytes are stained red; ×20 objective; scale bar = 100 um. c, d Quantitation of Masson’s trichrome, performed as described in “Methods”. a, c There is significantly increased collagen in PyMT/Col1a1 compared to PyMT tumors. Celecoxib (Clxb) diminishes collagen deposition in both PyMT and PyMT/Col1a1 tumors. b, d There is no significant effect of treatment with celecoxib in normal, tumor-free mammary glands. Glands in Col1a1 animals tend to have greater collagen accumulation than their wild-type counterparts. Raw data are depicted; **p < 0.01, p < 0.0001; n = 5 mice per arm for a and c and n = 1 mouse per arm for b and d; a and b are separate animal cohorts, age is 14 weeks for both animal cohorts; at least 5 image fields analyzed per 2–3 tumors/glands per animal; mixed linear model. Veh vehicle