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Fig. 2 | Breast Cancer Research

Fig. 2

From: Selective isolation and characterization of primary cells from normal breast and tumors reveal plasticity of adipose derived stem cells

Fig. 2

Immunofluorescence staining of cell isolates. a Immunofluorescence hybridization demonstrated the epithelial origin of cell isolates incubated with a pan-cytokeratin (KRT) type 5, 6, 8, 17 and 19 antibody. All epithelial cell fractions had positive localization of the cytoplasm (green). In contrast, in the mesenchymal cell fraction very few positive cells were identified (e.g., normal mammary cells (NORMA)3). Adipose-derived stem cells (ADSC) were KRT-negative. Nuclei were identified using 4',6-diamidino-2-phenylindole (DAPI) (blue). b CD90 immunocytochemical analysis was performed for mesenchymal cell characterization. Positive localization was visible in ADSC and mammary mesenchymal cell (MES) fractions (red). In contrast, epithelial cells were largely CD90-negative. Only in NORMA1 were a few rounded-shape cell colonies sparsely positive. Counterstaining was performed using DAPI (blue). c GATA-3 was visualized in epithelial cell isolates using immunocytochemical analysis. Almost all cells in the epithelial cell fraction expressed this transcription factor but it was not detected either in the MES or ADSC fraction (green). Counterstaining was performed using DAPI (blue). Overlay demonstrates co-localized cells with turquoise nuclei. Bar 100 μm

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