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Fig. 1 | Breast Cancer Research

Fig. 1

From: p53 deficiency linked to B cell translocation gene 2 (BTG2) loss enhances metastatic potential by promoting tumor growth in primary and metastatic sites in patient-derived xenograft (PDX) models of triple-negative breast cancer

Fig. 1

p53 silencing enhances breast tumor growth and metastasis in vivo. BC3-p53WT and BC3-p53KD cells expressing CBR-luc and mCherry were implanted into the humanized mammary fat pads of NOD/SCID mice. Bioluminescence imaging (BLI) was performed to monitor tumor growth and metastasis as a function of time. a Representative images showing growth and metastasis of BC3-p53WT and BC3-p53KD tumors. Due to rapid tumor growth BC3-53KD tumors were resected at approximately 9 weeks post-engraftment. b Total photon flux from each mammary tumor was quantified from BLI, and values were plotted as a function of time. c Tumors were measured with calipers, and values were plotted as a function of time. n = 18, BC3-p53WT; n = 18, BC3-p53KD. p = 0.004, t test (b and c). d and e 5 weeks and 9 weeks post-engraftment, tumors were harvested, formalin-fixed, sectioned, and stained with antibodies against phospho-histone H3 (pHH3) (d) and cleaved caspase 3 (CC3) (e). Positive regions were counted manually, and percentage of positive cells was calculated from DAPI-stained nuclei. Each data point is the average of at least six images from an individual mouse. Paired t tests, p = 0.01 for pHH3 at 5 weeks; p = 0.30 for pHH3 at 9 weeks; p = 0.04 for CC3 at 5 weeks; p = 0.20 for CC3 at 9 weeks. f BLI was performed biweekly to detect the appearance of lymph node metastases. p = 0.001, Wilcoxon rank sum test. Each data point represents one mouse. All error bars represent standard error of the mean (SEM)

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