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Fig. 7 | Breast Cancer Research

Fig. 7

From: Targeted Pten deletion plus p53-R270H mutation in mouse mammary epithelium induces aggressive claudin-low and basal-like breast cancer

Fig. 7

WAP-Cre:Ptenfl/fl:p53R270H/wt spindle and PDA tumor cells exhibit higher metastatic potential than WAP-Cre:Ptenfl/fl:p53fl/fl spindle tumor cells. a Kaplan-Meier survival curve of NOD/SCID female mice following tail vein injection with indicated primary tumor cells showing that p53R270H mutation accelerated metastatic formation relative to p53 deletion. Mice were euthanized when moribund. Statistical significance by Wilcoxon method: spindle Ptenfl/fl/p53fl/fl vs. spindle Ptenfl/fl/p53R270H, p = 0.0004; spindle Ptenfl/fl/p53fl/fl vs. PDA Ptenfl/fl/p53R270H, p = 0.0012; spindle Ptenfl/fl/p53R270H vs. PDA Ptenfl/fl/p53R270H, p = 0.16. b-e. Representative whole lung (b) and H&E images (c) of mice injected with WAP- Cre:Ptenfl/fl:p53R270H/wt mammary tumor cells at end point. Arrowheads indicate metastatic lesions. Representative H&E images of (d) primary spindle and (e) primary PDA WAP-Cre:Ptenfl/fl:p53R270H/wt mammary tumors and their metastatic counterparts. Note that one of the metastatic lesions from the PDA had spindle-shaped morphology (bottom right). f Representative images of E-cadherin expression in primary WAP-Cre:Ptenfl/fl:p53R270H/wt and WAP-Cre:Ptenfl/fl:p53fl/fl mammary tumors and lung metastases. g Expression levels of epithelial (E-cadherin) and mesenchymal (vimentin) markers in primary WAP-Cre:Ptenfl/fl:p53R270H/wt and WAP-Cre:Ptenfl/fl:p53fl/fl mammary tumors and lung metastases. H&E hematoxylin and eosin, PDA poorly differentiated adenocarcinoma

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