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Fig. 7 | Breast Cancer Research

Fig. 7

From: ELF5 isoform expression is tissue-specific and significantly altered in cancer

Fig. 7

E74-like factor 5 (ELF5) isoforms have a similar transcriptional effect in T47D and MDA-MB-231 cell lines. a ELF5 expression measured by quantitative polymerase chain reaction (qPCR) at 48 h for T47D clonal cell lines (top) and MDA-MB-231 clonal cell lines (bottom). Assay detects all ELF5 isoforms. Values are the mean calibrated normalized relative quantity (CNRQ) with standard error. Results relative to the minus doxycycline (−Dox) control (set at 1) for each cell line. b ELF5 expression measured by qPCR at 48 h for T47D clonal cell lines (top) and MDA-MB-231 clonal cell lines (bottom) used in the qPCR panel. Assay detects all ELF5 isoforms. Values are the mean CNRQ with standard error. Results relative to the sample with the lowest ELF5 value (set at 1), which is T47D-ELF5-isoform 2-V5 clone 8 (T47D lines) and MDA-MB-231-ELF5-isoform 3-V5 clone 22 (MDA-MB-231 lines). c Heat map showing genes (from 116-gene qPCR panel) with absolute fold change >3 (any T47D line) or >2 (any MDA-MB-231 line). Two clonal cell lines were tested per group. All heat maps use the legend shown in (e) and represent the log10 fold change (capped at −1 and +1) of the plus Dox quantity compared with the minus Dox quantity as measured by qPCR. Gray indicates gene was not detectable by qPCR in minus and/or plus Dox samples. d Functional categorization of selected genes from 116-gene qPCR panel. Some genes are represented more than once due to multiple functions. e Heat map showing genes (from 27-gene qPCR panel) with absolute fold change >3 (any T47D line) or >2 (any MDA-MB-231 line). Results shown for three or four clonal lines per group. f Functional categorization of selected genes from 27-gene qPCR panel. g Heat map representing the mean log10 fold change per group for all genes in the 27-gene panel, as well as ELF5. Significant p values are shown where false discovery rate (FDR) is <0.10. Some p values (nonbold) are >0.05, although FDR for these values is <0.10. Nonsignificant p values (FDR >0.10) are not shown

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