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Fig. 5 | Breast Cancer Research

Fig. 5

From: PTK6 inhibition promotes apoptosis of Lapatinib-resistant Her2+ breast cancer cells by inducing Bim

Fig. 5

Protein tyrosine kinase 6 (PTK6) regulates Bim in part through p38 mitogen-activated protein kinase (MAPK) activation. a UACC893R1 or MDA-MB-453 cells expressing either control or PTK6 shRNA (49, C9 or 12) were lysed. Lysates were probed with indicated antibodies. b UACC893R1 or MDA-MB-453 cells expressing either control or PTK6 shRNA (49, C9 or 12) were cultured in the presence of dimethyl sulfoxide (DMSO) or SB203580 (p38 inhibitor). Cells were lysed for 72 h (UACC893R1) or 96 h (MDA-MB-453) after infection, and lysates were probed with the indicated antibodies. Immunoprecipitation and western blot analysis were performed to show PTK6 downregulation in UACC893R1 cells; *PTK6 that was immunoprecipitated. c Cells expressing either control or PTK6 shRNAs (49, C9 or 12) were treated with either DMSO or SB203580. Cells were lysed 72 h (UACC893R1) or 120 h (MDA-MB-453) after shRNA lentiviral infection. Lysates were probed with the indicated antibodies. Experiments were performed three times

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