Fig. 3From: PTK6 inhibition promotes apoptosis of Lapatinib-resistant Her2+ breast cancer cells by inducing BimProtein tyrosine kinase 6 (PTK6) downregulation induces apoptosis of Lapatinib-resistant human epidermal growth factor receptor 2 (Her2)+ breast cancer cells. a MDA-MB-453 and UACC893R1 cells expressing control or two different PTK6 shRNA (C9 and 49) were fixed, stained with propium iodide (PI), and analyzed by flow cytometry. The percentage of cells in the sub-G1 population is plotted. b MDA-MB-453 and UACC893R1 expressing control or PTK6 shRNA (C9 or 49, respectively for the two cell lines) were stained with Annexin-V and PI, and analyzed by fluorescence-activated cell sorting. c Cells expressing control or two different PTK6 shRNAs (C9, 49) were lysed 96 h (MDA-MB-453) or 72 h (UACC893R1) following infection with shRNA lentivirus. Lysates were probed with indicated antibodies. Immunoprecipitation and western blot analysis confirms PTK6 downregulation in UACC893R1 cells. Figures are representative of three independent experimentsBack to article page