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Fig. 2 | Breast Cancer Research

Fig. 2

From: PTK6 inhibition promotes apoptosis of Lapatinib-resistant Her2+ breast cancer cells by inducing Bim

Fig. 2

Protein tyrosine kinase 6 (PTK6) downregulation inhibits growth of Lapatinib-resistant human epidermal growth factor receptor 2 (Her2)+ breast cancer cells. a UACC893R1 or MDA-MB-453 cells grown in monolayer cultures expressing control or PTK6 shRNA (C9), were counted at the indicated number of days. b Cells expressing either control or two different PTK6 shRNAs (C9, 49) were grown in 3-D MatrigelTM cultures. Cells were re-fed with fresh media every 3–4 days. The figure represents day-16 cultures (UACC893R1) or day-23 cultures (MDA-MB-453). Scale bar 30 μm. c Cells expressing control or PTK6 shRNA (C9, 49) were cultured in soft agar assays for 30 days. Colonies greater than 50 square pixels were counted and plotted. Downregulation of PTK6 was confirmed by western analyses for MDA-MB-453 and UACC893R1 cells. d UACC893R1 cells expressing control or PTK6 shRNA (C9) were injected subcutaneously into flanks of 6-week-old female nude mice (n = 5/group). Tumor size was measured and recorded every 3–4 days. All figures are representative of three independent experiments except for the xenograft study, which was performed twice; *p <0.05; **p <0.005

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