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Fig. 4 | Breast Cancer Research

Fig. 4

From: MicroRNA profiling of the pubertal mouse mammary gland identifies miR-184 as a candidate breast tumour suppressor gene

Fig. 4

miR-184 represses various substrates in the AKT/mTOR signaling pathway. a Analysis of miR-184 seed matches in the promoter, 5′ UTR, open reading frame (ORF) and 3′ UTR of miR-184 downregulated and upregulated transcript reveals strong enrichment for miR-184 seed matches in the 5′ and 3′ UTRs of downregulated genes. The Wilcoxon rank-sum test was used to calculate p values. b Distribution of gene expression changes in genes with miR-184 seed regions in either 3′ UTR or 5′ UTR only. c Distribution of gene expression changes in genes with miR-184 seed regions in either 5′ UTR or 3′ and 5′ UTR only. d Immunoblot of total AKT2, PRAS40, GSK3A and Actin in MDA-MB-231 transfected with negative (neg) control or miR-184 mimics for 48 h. e Luciferase 3′ UTR reporter assay performed in HEK293T confirmed that CSF1, GSK3A, AKT2 and PRAS40 are direct targets of miR-184. ITGB1 is a negative control and a reporter containing 3′ UTR sequences perfectly complementary to mIR-184 (PMR) serves as a positive control

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