Figure 2

The RCA-I-binding tendency is constant with the invasion ability of TNBC cells rather than those of non-TNBC cells. (a) FCM analysis confirms the proportional, inversely proportional, and uncorrelated binding to the metastatic capacity of these cells with RCA-I, WGA, and WFA, respectively. (b) Incubation of biotinylated RCA-I, WFA, or WGA, followed by the addition of Cy3-conjugated streptavidin and direct inspection with microscopy further confirmed the cell-binding tendencies of these lectins. (c) The RCA-I-binding positive rates of five TNBC (red) and two non-TNBC cells (green) were analyzed by FCM, which were referred to their relative metastatic abilities. (d) The immunofluorescent staining of RCA-I binding to another two TNBC cells (SUM159 and BT549) and two non-TNBC cells (MCF7 and SKBR3). FCM, flow cytometry; RCA-I, Ricinus communis agglutinin I; TNBC, triple-negative breast cancer; WFA, Wisteria floribunda agglutinin; WGA, Triticum vulgaris agglutinin.