Figure 6

The effect of siRNA (CHKA) on cell cycle as determined by flow cytometry. (A) Flow cytometry was used to compare DNA content in Sum44 WT cells treated with dextran charcoal-stripped media (DCC) and DCC + E2. There was no significant difference between the cell cycle phases in the cells treated with DCC. CHKA arrests cells at the G1/S, but not at the G2/M phase of the cell cycle in cells treated with DCC + E2. (B) The percentage of cells at each cell phase: G0/G1, S, G2/M are shown on the bar chart as the mean ± SD. The experiment was performed in triplicate; **P <0.005 and *P <0.01.