Figure 6

Elevated BRCA1-IRIS and survivin and lack of FOXO3a expression correlates with breast tumors aggressiveness. Paraffin-embedded tissue microarray sections were examined by immunohistochemistry with anti-BRCA1-IRIS, survivin and FOXO3a mAb. (A and B) BRCA1-IRIS and survivin, respectively staining scores in normal (n = 66), DCIS (n = 167), invasive (n = 179) and metastatic (n = 99) breast cancer tissue samples. (C and D) BRCA1-IRIS and survivin, respectively staining scores per field as compared to the localization of FOXO3a in each cell in normal (n = 66), DCIS (n = 167), invasive (n = 179) and metastatic (n = 99) breast cancer tissue samples. (E) Percentage of probability of disease-free survival in patients with TNBC tumors overexpressing low (blue line) vs. middle/high (red line) levels of EGFR/AKT/MDM2/Skp2/survivin as a surrogate for BRCA-IRIS overexpression. DCIS, ductal carcinoma in situ; FOXO3a, Forkhead box class OAKT3a; mAb, monoclonal antibody; TNBC, triple negative breast cancer.