Skip to main content
Figure 8 | Breast Cancer Research

Figure 8

From: A co-culture genome-wide RNAi screen with mammary epithelial cells reveals transmembrane signals required for growth and differentiation

Figure 8

Genes modulating 184- hTERT -L9 co-culture growth also affect primary human mammary epithelial cell growth. Single-cell suspensions of dissociated human primary mammary cells were infected with individual short-hairpin RNA (shRNA) constructs targeting our screen-identified gene set prior to being plated in colony-forming assays. The fraction of surviving colonies after silencing of the target genes relative to a nontargeting shRNA control is plotted. Error bars represent 95% confidence intervals (n = 3 patient samples).

Back to article page