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Figure 6 | Breast Cancer Research

Figure 6

From: DEAD-box protein p68 is regulated by β-catenin/transcription factor 4 to maintain a positive feedback loop in control of breast cancer progression

Figure 6

Wnt signaling-mediated p68 upregulation is important in the induction of EMT. (a) 4T1 cells were transfected with either β-catenin or transcription factor 4 (TCF4). After 36 h, lysates were prepared and analysed for epithelial to mesenchymal transition (EMT) marker proteins by immunoblotting (IB). (b) MDA-MB 231 and 4T1 cells were transfected with scrambled, tcf4 and p68 small interfering RNAs (siRNAs) independently. After 48 h, whole cell lysates (WCLs) were prepared and analysed for EMT marker proteins by IB. (c) Wnt3a-MCF7 and empty vector (EV)-MCF7 (control) stable cells were analysed for their anchorage-independent growth by formation of soft-agar colonies. Quantification of the number of colonies and error bars are represented in the figure. Standard deviation (SD) was calculated from three independent biological repeats. Statistical significance was analysed by Student’s t test (P <0.001) (top). Colony-forming ability of Wnt3a-MCF7 and EV-MCF7 cells were determined after individual knockdown of β-catenin, tcf4 and p68 using respective siRNAs as depicted in the figure (bottom). (d) Wnt3a-MCF7 and EV-MCF7 (control) stable cells were analysed for their invasiveness using matrigel invasion assay. Error bars are represented in the figure. SD was calculated from three independent biological repeats. Statistical significance was analysed by Student’s t test (P <0.001).

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