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Figure 6 | Breast Cancer Research

Figure 6

From: Bone marrow stromal antigen 2 expressed in cancer cells promotes mammary tumor growth and metastasis

Figure 6

Suppression of BST-2 expression in cancer cells results in anchorage-independency. (A and C) Quantification of the number of colonies formed in soft agar by BST-2-expressing shControl, BST-2-suppressed sh137 and sh413 murine cells, MCF-7 (positive control), HMLE and NMuMG cells following a 20-day transformation assay. The bar represents number of colonies per field (n = 5) for each cell type. Error bars correspond to standard deviations. Significance was taken at P <0.001**. (B and D) Representative images of crystal violet-stained colonies from a soft agar assay showing anchorage-independent growth of cancer cells. Clones were imaged at 4X. Experiments were repeated multiple times with similar results. ns = not significant.

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