Figure 1
Hormones are necessary for luminal tumor metastases. (A) Ovariectomized (Ovx’d) non-obese diabetic/severe combined immunodeficient gamma (NSG) mice were intracardiac (IC)-injected with 105 luciferase and ZsGreen-tagged basal-like estrogen withdrawn-line 8 (EWD8) or luminal E3 cells, and implanted with cellulose control (C) or with estrogen (E) or estrogen + progestin (E+P)-releasing pellets. Weekly quantitation used in vivo bioluminescent imaging (BLI). Data are presented as mean ± standard error of the mean (SEM) of BLI signal (n = 20 per treatment group). *P <0.05, **P <0.005, Student’s t test. (B) Representative luciferase BLI whole-body images. (C) Kaplan-Meier survival curves (n = 20 per treatment group); P = 0.003, log-rank test. (D) Ovx’d NSG mice were IC injected with 5 × 105 luciferase and ZsGreen-tagged luminal MCF-7 cells and implanted with C, E, or E+P pellets. Weekly in vivo luciferase BLI quantitation. Data are presented as mean ± SEM of BLI signal (n = 20 per treatment group). *P <0.05, Student’s t test.