Figure 5
From: Tumor-expressed adrenomedullin accelerates breast cancer bone metastasis
Small-molecule antagonists block adrenomedullin-induced bone formation. Two small molecules blocked adrenomedullin (AM)-induced bone formation. Neonatal mouse calvarial bones were cultured in triplicate for 7 days and treated with 10 nM AM ± 1 μM inhibitor NSC 16311 (A) or NSC 37133 (B). Sections were assessed for new bone formation, expressed as area in histological sections, indicated on the vertical axis, by quantitative histomorphometry. ***P < 0.001. An example of the assay is provided in Additional file 1: Figure S1. (C) Chemical structures of the NSC compounds are shown [47].