Skip to main content
Figure 5 | Breast Cancer Research

Figure 5

From: Trefoil factor 3 promotes metastatic seeding and predicts poor survival outcome of patients with mammary carcinoma

Figure 5

Forced expression of TFF3 in MCF7 cells increased phosphorylation of STAT3 to promote invasion; and TFF3 expression significantly correlates with pSTAT3 expression in MC patients' tissue. (A) Western blot analysis was used to assess the levels of pSTAT3 and STAT3 in MCF7 cells with either forced or depleted expression of TFF3 as described in Methods. (B) STAT3 mediated transcription, α2-M promoter activity in MCF7 cells with either forced or depleted expression of TFF3, on exposure to JSI-124 (0.2 μM) or Stattic (2 μM) inhibitor; and transiently transfected with STAT3 DN, STAT3-siRNA or STAT3 CA. The luciferase assay was performed as described in Methods. (C) CDH1 promoter activity in MCF7 cells with either forced or depleted expression of TFF3, on exposure to JSI-124 (0.2 μM) or Stattic (2 μM) inhibitor. The luciferase assay was performed as described in Methods. (D) Western blot analysis was used to assess the levels of CDH1 in MCF7 cells with forced expression of TFF3 on exposure to JSI-124 (0.2 μM) or Stattic (2 μM) inhibitor as described in Methods. (E) Invasive capacity of MCF7 cells with either forced or depleted expression of TFF3, on exposure to JSI-124 (0.2 μM) or Stattic (2 μM); and transiently transfected STAT3 DN, STAT3-siRNA or STAT3 CA. Cell invasion was evaluated using a Transwell assay. (F) Correlation between TFF3 expression and pSTAT3 in the MC cohort utilized in Figure 1B. Pearson's chi-square test was used to compare the differences between groups. Statistical significance was assessed by using an unpaired two-tailed Student's t test (P <0.05 was considered as significant) using GraphPad Prism 5. Columns are mean of triplicate experiments; bars, ± SD. **P <0.001, *P <0.05.

Back to article page