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Figure 2 | Breast Cancer Research

Figure 2

From: Modeling luminal breast cancer heterogeneity: combination therapy to suppress a hormone receptor-negative, cytokeratin 5-positive subpopulation in luminal disease

Figure 2

Pure luminal (pLUM) suppress proliferation of neighboring pure luminobasal (pLB) in mixed-cell xenografted solid tumors. (A) Whole-tumor cross-sections of untagged pLUM, ZsGreen (ZsG)-pLB and 5:1 pLUM:pLB xenografts grown in ovariectomized mice without hormones. Tumor sections counterstained with 4′,6-diamidino-2-phenylindole (DAPI) (blue). Scale bars equal 2000 μM. (B) Quantitation of ZsG fluorescence in pLUM, pLB and 5:1 pLUM:ZsG-pLB tumors relative to fluorescence of pure ZsG-pLB tumors under control (C) or estrogen (E) conditions. (C) Proliferation rates quantified by bromodeoxyuridine (BrdU) incorporation of pLUM and ZsG-pLB subpopulations in pure and mixed-cell tumor xenografts. Percent BrdU + cells in CK5– and CK5+ cells were quantified for ≥5 independent tumors; *P ≤0.05.

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Breast Cancer Research

ISSN: 1465-542X

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