Figure 5

Nestin silencing inhibits the Wnt/β-catenin signaling in breast CSC. Nestin-si and Nestin-c breast CSC were cultured for 48 h, and the relative levels of target proteins and phosphorylation were determined by western blotting. Data are presented as the mean ± SD of the levels of target proteins vs. the β-actin control, or the phosphorylated vs. the total form, from six separate experiments. In addition, Nestin^high in the presence or absence of LGK974 (1 nM), Nestin^low in the presence or absence of SB216763 (5 μM), and control CSC were tested for their proliferation via mammosphere formation assays. Western blot analysis. (A-C). Quantitative analysis of the relative levels of target proteins. (D) Quantitative analysis of the formed mammospheres. ^*P <0.05, vs. the Nestin-c or control, except for specifically indicated. CSC, cancer stem cell; SD, standard deviation.