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Figure 2 | Breast Cancer Research

Figure 2

From: Clonogenic growth of human breast cancer cells co-cultured in direct contact with serum-activated fibroblasts

Figure 2

Results of the co-culture assay. Each bar represents the mean and standard deviation of triplicate measurements. Tumor cells seeded on a monolayer of serum-activated fibroblasts produced the highest average number of colonies. There were significantly fewer colonies if fibroblasts or serum were omitted. Co-culture with fibroblasts separated by permeable transwell inserts or on a monolayer of B16 cells yielded low numbers of colonies that were statistically indistinguishable from the control using tumor cells grown in serum-supplemented medium without fibroblasts.

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