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Figure 3 | Breast Cancer Research

Figure 3

From: Claudin 7 expression and localization in the normal murine mammary gland and murine mammary tumors

Figure 3

Immunolocalization of claudin 7 (Cy3, red) in cultured mammary epithelial cells, epididymis, lung, kidney, and liver. ZO-1 is stained with fluorescein isothiocyanate to demonstrate location of the tight junctions. (a) Eph4 cells. Claudin 7 is found with a patchy distribution at the tight junctions with ZO1. Vesicular stain can also be seen in the cytoplasm. The inset at the top shows the z axis across the xy view of the section at the level of the arrow. The overlap of ZO1 and claudin indicates colocalization at the level of resolution of the light microscope. (b) Mouse epididymis. Claudin 7 stain colocalizes with ZO-1 in most, but not all, tight junctions. Lu, lumen. Small arrowheads in this and subsequent panels denote the apical border of the epithelium. (c) Lung. The luminal cells of the bronchiole show heavy staining for claudin 7 in a punctate distribution at the basolateral surface of the cell; however, some claudin 7 colocalizes with ZO-1 (see black-and-white inset). (d) Kidney. Some of the tubules of the renal cortex stain as observed by Li and colleagues [7]. Claudin 7 shows a punctate distribution that is heaviest in the basal portion of the cells. (e) Liver. Tight junctions at the bile canaliculi are stained with ZO-1. However, no stain for claudin 7 is apparent. The magnification for each figure is indicated by the label (u, μm) above the scale bar.

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