Figure 2From: Knockdown of c-Myc expression by RNAi inhibits MCF-7 breast tumor cells growth in vitro and in vivoTime course of the reduction in c-Myc protein levels by pSilencer–c-Myc. Exponentially proliferating MCF-7 cells were transfected with pSilencer–c-Myc or pSilencer and whole cell lysates were prepared at the time points indicated. Total cell lysates were separated by SDS–polyacrylamide-gel electrophoresis and immunoblotted with an antibody against c-Myc; expression levels were normalized for loading by probing for β-actin.Back to article page