Figure 7
Signaling pathway inhibitors differentially affect cell proliferation. 17β-Estradiol (E2; 10 nmol/l) was introduced on day 0. Adenylyl cyclase stimulator (forskolin; 10 μmol/l) and cAMP analog (8-CPT cAMP; 250 μmol/l) were replenished every second day. PKA inhibitor (H-89; 10 μmol/l) and adenylyl cyclase inhibitor (SQ 22,536; 300 μmol/l) were applied at day 0 together with 10 nmol/l E2. Cells were fixed on days 1, 3 and 5, and their number estimated by crystal violet (CV) assay. The data are averaged values ± standard error (error ranges were very small and are contained within the size of the symbol) of two separate experiments, each containing 24 replicates. mERhigh, MCF-7 cells enriched for membrane estrogen receptor-α; mERlow, MCF-7 cells depleted for membrane estrogen receptor-α.