Human breast | Thrombin | Endothelin B | Â | Â | Â | Â | Â | Â | Â |
---|
cancer cells | receptor | receptor | TIE-2 | CD31 | Endoglin | Angiopoietin-1 | VEGF | L32 | GAPDH |
---|
HUVEC | ++ | ++*
| +++ | ++ | ++ | ++ | ++ | ++ | ++ |
MCF-7 | - | - | - | - | - | - | + | ++ | ++ |
MB-231 | ++ | ++ | + | + | - | - | +++ | ++ | ++ |
- Comparison of qualitative expression assessment of vascular markers by human breast cancer cells MCF-7 (poorly aggressive), MDA-MB-231 (MB-231; highly aggressive) and HUVECs, as shown in the raw data from the ribonuclease protection assay and differential display analysis in Figure 1. Equal loading of experimental samples was achieved by ribosomal protein L32 (L32) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) control lanes. *Data not shown.