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Table 2 Summary of transforming growth factor-β1-induced cellular responses in vitro

From: Induction by transforming growth factor-β1 of epithelial to mesenchymal transition is a rare event in vitro

 

TGF-β1-induced responsea

Cell strain

Acquisition of spindle shapeb

Loss of junctional E-cadherinc

Loss of junctional ZO-1c

Actin stress fiber formationc

HEK

±

-

N/Ad

±

HMECe

±

-

N/Ad

+

HaCaT

-

-

-

±

Colo357

-

-

-

±

Panc-1

-

-

-

-

NMuMG

+

+

+

+

MCT

+

+

+

+

  1. aTreatment with 5 ng/ml transforming growth factor (TGF)-β1 for 48 hours. bDetermined by phase contrast microscopy. cDetermined by E-cadherin, zonula occludens (ZO)-1, or F-actin immunofluorescence. dHuman epidermal keratinocytes (HEKs) and human mammary epithelial cells (HMECs) exhibited little to no junctional ZO-1 staining in the absence of TGF-β1 treatment. eHMECs from patients #1012 and #1016. Cellular responses to TGF-β1 designated by + (strong), ± (weak), or - (negative).