Peritoneal/venous shunts of ascitic fluid [14] | |||
---|---|---|---|
Site of primary tumor | Average number of viable cells per 20 ml blood (delivered on average for 40 weeks) | Average clonogenic efficiency in soft agar | Metastatic events in patients at autopsy |
Ovary, breast, pancreas bronchus, and colon | 1 × 107 | 1 in 104 | 8/15: no viable cells 7/15: large number of single viable tumor cells and micrometastases in lung only |
Cell types | Gene expressed | Number of cells per injection | Lung colony efficiency |
NIH-3T3 [16] | c-H-ras | 3 × 105 | 1 in 104 |
 | v-src | 3 × 105 | 2 in 105 |
MDA-MB-231 [17] | - | 1.0 × 106 | <1 in 105 |
MDA-MB-435 [17] | - | 1.0 × 106 | <1 in 105 |